Journal: Reproductive Biology and Endocrinology : RB&E
Article Title: Co-culturing of follicles with interstitial cells in collagen gel reproduce follicular development accompanied with theca cell layer formation
doi: 10.1186/1477-7827-9-159
Figure Lengend Snippet: Immunohistochemical localization of the cell markers in mouse ovaries . Immunohistochemistry of (a) Flk-1, (b) F4/80, (c) fibronectin, (d) laminin, (e) tenascin, (f) collagen type IV, (g) THY1, and (h) CYP 17A-1 in mouse ovaries. (a, b, d-h) visualized by immunofluorescence and (c) visualized by ABC staining. O, oocyte; G, granulosa cells; T, theca cells. Scale bars = 25 μm.
Article Snippet: Inc, Mill Creek, WA, USA), goat anti-mouse Flk-1 antibody (R&D Systems, Inc, Minneapolis, MN, USA), rat anti-mouse F4/80 antibody (Serotec Morphosys UK Ltd, Oxford UK), goat anti-mouse CYP17A-1 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rat anti-mouse THY1 antibody (Biomeda, Foster City, CA, USA), rabbit anti-mouse collagen type IV antibody (Cosmo Bio Co., Ltd., Tokyo, Japan), and rat anti-mouse tenascin antibody (Sigma-Aldrich Corporation, St. Louis, MO, USA).
Techniques: Immunohistochemical staining, Immunohistochemistry, Immunofluorescence, Staining